The aim of this work was to perform chemical and physicochemical analysis of oil of the camu camu (Myrciaria dubia (H.B.K) Mc Vaugh) seeds obtained at Caçari beach, on the banks of the Cauamé river, in Boa Vista city, Roraima state, Brazil. From the seeds, the dark green oil was 2.88% yield. The physical and chemicalproperties of the oil through the 1H and 13C NMR spectra: iodine content (55.39 g I2 100g-1), saponificationindex (178.14 mg KOH g-1), acidity (1.46 mg KOH g-1), olefinic/aliphatic hydrogen ratio (0.36), average molecular weight (934.16 g mol-1). Analysis of the signal displacements in 1H and 13C NMR spectra confirmed the presence of the fatty acid functional groups and unsaturated fatty acid unsaturations, so the concentrationof linolenic acid was 0.43%, acid 12.02% oleic, oleic was 62.49% and saturated fatty acids 25.10%. The quantitative analysis of minerals by ICP-OES showed concentrations of elements: K, Na, P, Si (> 100 ppm); Mg, S (10 to 100 ppm) and Ti (<100 ppm). The antioxidant activity, through the DPPH method, shows thatcamu camu oil has a high antioxidant percentage (IC50 = 114.72 µg mL-1).