In this paper, the electrostatic interaction between carbon nanotubes with electronegative surface treated with polyacrylic acid and the hydrotalcite with positively charged laminate is used to and the ZnAl-LDH in-situ growth is installed onto the surface of carbon nanotubes to obtain the hydrotalcite/carbon nanotube composite. The analytical ability of different fluorescein intercalated LDH-PCNT composite modified electrodes for dopamine is studied and the results show that the pH value has a significant impact on the fluorescein intercalated hydrotalcite. The fluorescein in the complex is not only inserted into the hydrotalcite interlayer, but the intercalation composite is well integrated with the carbon nanotubes. Due to the accelerant role of the fluorescein and the carrier-mediated role of carbon nanotubes, the electrocatalytic ability of the composite is improved and the dopamine has a low detection limit on the surface of modified electrodes. The preparation method of the composite is simple and the electrocatalytic performance is good. The analyte has a low detection limit and high sensitivity on modified electrodes, which greatly expands the assembly method and application range of the electrochemical sensor.