Lowering the costs of industrial fermentation processes is a current challenge of modern biotechnology. In this sense, has been studied the possibility to replace the conventional medium for vitamin B2 abd B12 biosynthesis with new effective medium from renuable plant-based sources. Riboflavin (vitamin B2) and cobalamin (vitamin B12) are essencial compounds in humans diets and their demand is increasing globally. Current manufacturing process of these vitamins is unsustainable because of using costly unreneable resources and generating hazardous wastes. Meat peptone is conventionally used as a nitrogen source during the fermentation process, however it is relatively expencive. Sunflower protein is a secondary product, which can be obtained from sunflower meal after oil extraction, and rich on its nutritional value. The objective of this study was to select and evaluate fermentative potential of sunflower protein and its enzymatic hydrolysates as a nitrogen source for riboflavin and cobalamin biosynthesis by evaluating the kinetic parameters of strains growth, specific substrate consumprion and formation of metabolites. The fermentation of strains was carried out using conventional mineral medium with meat peptone in 250 mL Erlenmeyer flasks, and the results obtained were compared with fermentation where sunflower protein and it’s enzymatic hydrolysates were used as a nitrogen source instead of meat peptone. It was observed that when compared with meat peptone, some strains show positive effect in growth when nitrogen source was substituted with sunflower protein enzymatic hydrolysates. This allows affirming that sunflower protein and its enzymatic hydrolysates are promising in the use as a nitrogen sourse for riboflavin and cobalamin biosynthesis. The characteristics and parameters of studied fermentation processes reveals their potential for residue products applications for obtaining products with high added value.