Gelatin and carboxymethylcellose from waste product were selected for wound replacement application. Carbodiimide was used to strengthen the scaffold structure which made from gelatin mixed with carboxymethylcellulose (CMC) using freeze drying method. The mixed scaffold was fabricated in various gelatin/CMC ratios which were 100/0, 90/10, 80/20 and 70/30, respectively. The mechanical characterization of the scaffold was done by using universal testing machine (UTM) to determine compressive modulus. The results showed the highest value compressive modulus which was from gelatin/CMC at ratio of 70/30 with 4.80±1.95 kPa. The compressive modulus from gelatin/CMC at ratio of 80/20 showed the lowest value with 1.48±0.58 kPa. The 3D Finite element analysis could predict stress distribution and shear stress that were consistency with experimental data. It shown deformation behavior of the scaffold which was the similar trend with the experimental result in every case. The strain energy from finite element analysis of 70/30 scaffold showed the highest value. From the results, this condition of scaffold could be used for wound replacement applications.