In recent years, the presence of antibiotics in the environment has become a new threat to living organisms. Many advanced treatment technologies have been applied to treat antibiotic residues, especially in advanced oxidation processes. The traditional wastewater treatment process can be replaced by advanced oxidation (AOP), highly reactive free radicals, especially hydroxyl radicals (•OH) generated via Fenton (H2O2/Fe), peroxone (O3/H2O2), electrochemical oxidation, photochemical, serve as the main oxidant. In this study, antibiotic residues of Fluoroquinolones (Ofloxacin) are assessed in hospital wastewater after treatment and evaluated factors affecting peroxone oxidation process. The results show that the treatment efficiency is 72 % at initial OFL concentration of 20 µg.L-1, O3 (5 mg.L-1) flow rate is 1 L.min-1, initial H2O2 concentration of 50 mg.L-1 at pH 7. The OFL removal efficiency by peroxone process decreased when inorganic ions (CO32-, HCO3-, Cl-) were added at the concentration of 1mM. . The peroxone process is a promising technique to replace traditional technologies to treat antibiotic residues in wastewater.