In crop control, weed is considered as a harmful plant. Weed can be controlled by applying the biochemical compound from allelopathic plants, which are environmentally friendly. In this study, a type of allelopathic plant, Sembung Vine (Mikania micrantha) that grow in Bukit Lama Palembang, was processed as a bioherbicide. A phytotoxicity test was conducted on the plant extract in methanol and extract mixture of n-hexane: ethyl acetate. The test was applied on the mung bean (Vigna radiata) seeds that were grown on a petri dish to analyze the germination, seedlings length, inhibitory effect, and biomass weight for methanol extract at 5; 10; 15; 20 % (w/v). The crude methanol extract was fractionated with the mixture of n-hexane: ethyl acetate (1:0; 9:1; 8:2; 7:3; 6:4; and 0:1). Each sample was tested at 4,000 ppm crude extract concentration to determine phytotoxicity effects. All samples were in triplicates. The methanol extract showed the highest inhibition of 99.4 % at 20 % (w/v). The fraction of M.mircrantha extract with n-hexane: ethyl acetate eluent showed the highest inhibition of 55.48% at 6:4 ratio.