Analysis was conducted on the degradation of indoline present in LB (Luria Bertani broth) broth used as rich growth medium during the bioremoval of Pb(II) using an industrially obtained microbial consortium. Indoline is a saturated analogue of indole, which is a well-known environmental pollutant and health concern found in industrial and agricultural wastewater. A method for Indoline analysis was developed using reverse phase high performance liquid chromatography (HPLC) run isocratically with methanol and 0.1% TFA (Trifluoroacetic acid). Experiments were conducted anaerobically in the presence of both 80 ppm and 500 ppm Pb(II) and each presented complete degradation of indoline within 33 hours. The study focuses on the novel method developed for HPLC analysis of indoline found in LB broth and its degradation in the presence of Pb(II).