Graphene is a promising reinforced material used in a vasty of applications due its high mechanical resistance, high electrical and thermal conductivity. These properties could improve the electrical conductivity and mechanical properties of polymeric nanofibers. Nervous, muscular, and cardiac tissues are a challenge for Tissue Engineering not only because of their poor regeneration but because conventional, non-conductive scaffolds are unable to establish an adequate electromechanical coupling. In the present work, graphene was investigated as a nanofiller in a muti-layered polymer scaffold manufactured by electrospinning. The graphene-doped scaffolds were characterized to prospect their use in tissue engineering.