Modelling Mgco3 Buffered Succinic Acid Production by Actinobacillus Succinogenes
Sibiya, Nhlamulo G.
Mamabolo, Ditebogo M.
Greyling, Cara
Chirwa, Evans M. N.
Daramola, Michael
Mokwatlo, Sekgetho C.
Brink, Hendrik G.

How to Cite

Sibiya N.G., Mamabolo D.M., Greyling C., Chirwa E.M.N., Daramola M., Mokwatlo S.C., Brink H.G., 2022, Modelling Mgco3 Buffered Succinic Acid Production by Actinobacillus Succinogenes, Chemical Engineering Transactions, 94, 1435-1440.


The current study focused on using a previously published model known to accurately predict the cultivation of Actinobacillus succionogenes on glucose in a NaOH neutralised system, for the prediction of batch system behaviour when MgCO3 is used as a pH buffer in a batch reactor system. The use of MgCO3 as a neutralising agent resulted in a biomass growth lag of about 8 h, followed by a sharp increase untill 15 h at which point the biomass concentration remained constant throughout the fermentation run. The maximum biomass concentration was 3.62 g/L. The fermentation ceased at a final SA concentration of 38.12 g/L and the SA yield on glucose was 0.81 g/g from an initial glucose concentration of 60 g/L. At the end of the fermentation, 79 % of the glucose as consumed. A residual glucose concentration of 12.84 g/L at the maximum SA concentration indicated product-related inhibition. The biomass, acetic acid (AA), and formic acid (FA) concentrations were incorrectly predicted by the model which implies that the use of MgCO3 as a buffer agent changed the metabolic fluxes of the organism.