TY - JOUR AU - Grebennikova, Olga V. AU - Sulman, Aleksandrina M. AU - Matveeva, Valentina G. PY - 2022/09/01 Y2 - 2024/03/28 TI - Use of Magnetic Nanoparticles Fe3O4 in the Synthesis of Biocatalysts Based on Horseradish Root Peroxidase JF - Chemical Engineering Transactions VL - 94 SP - 673-678 SE - Research Articles DO - 10.3303/CET2294112 UR - https://www.cetjournal.it/index.php/cet/article/view/CET2294112 AB - In this study, magnetic nanoparticles Fe3O4synthesized by the polyol method were used to immobilize Horseradish root peroxidase. The surface of magnetic nanoparticles was pretreated with tetra-ethyl orthosilicate, 3-aminopropyltriethoxysilane, and glutaraldehyde. As a result, two biocatalysts were obtained Fe3O4/APTS/GA/HRP and Fe3O4/TEOS/APTS/GA/HRP. The activity of the synthesized biocatalysts was estimated spectrophotometrically in the oxidation reaction of 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) with hydrogen peroxide. The optimal conditions for this reaction were selected (pH 6.0, temperature 45 °C). It has been determined that biocatalysts Fe3O4/APTS/GA/HRP and Fe3O4/TEOS/APTS/GA/HRP slightly decreased their activity during 5 consecutive experiments, by 19 and 15 %. ER -