Biotechnological Production of Xylitol: Evaluation of Detoxification Process with Residual Lignin Using Response Surface Methodology
Santos Lima, F.C.
Silva, F.L.H.
Gomes, J.P.
Muniz, M.B.
Santiago, Â.M.
Silva, C.G.
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Santos Lima F., Silva F., Gomes J., Muniz M., Santiago Â.M., Silva C., 2014, Biotechnological Production of Xylitol: Evaluation of Detoxification Process with Residual Lignin Using Response Surface Methodology, Chemical Engineering Transactions, 38, 415-420.
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Abstract

The agroindustrial residues are among the greatest sources of biomass in the world which can be used in bioprocesses, because these lignocellulosic materials represent an abundant source of sugars. In northeastern Brazil one of the lignocellulosic residues in abundance, which has most of the potential wasted is the cashew apple bagasse; that through pretreatment with dilute acids makes possible the removal in hemicellulosic fraction (xylose, arabinose, glucose, mannose and galactose) which represent substrates that can be utilized for the production of xylitol by fermentation. However this process, besides the released sugars are also compounds fermentation inhibitors. In this study the main aim was to evaluate through response surface methodology detoxification treatment using residual lignin (cashew aplle bagasse which precipitates in the pretreatment liquor). The cashew apple bagasse was submitted to acid prehydrolysis H2S04 using 3% (w/w), was then carried out analyzes of sugars and inhibitory compounds present in the prehydrolyzate liquor in high efficiency liquid chromatography (HPLC). The prehydrolyzate was concentrated and then treated under various conditions of mass residual lignin, pH and contact time to reduce concentration toxic compounds and improve efficiency process to subsequent fermentation (production of xylitol). The results indicate that to obtain in the prehydrolyzate liquor, a higher percentage removal from HMF (Hydroxymetylfurfural) and furfural, it should operate under conditions of pH 4, 0.65 g mass of lignin for 20 min, removing approximately 100%.
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