The objective of this study was to evaluate the lipid content in the sludge produced by the biomass of Microcystis protocystis, obtained in the process of water treatment coagulation/flocculation/dissolved air flotation (C/F/DAF), using as coagulant Moringa oleifera Lam (MO) seeds. For the tests was used deionized water artificially contaminated with cell cultures M. protocystis, obtaining a cell density of 106 cells.mL-1. Cyanobacterium cells were grown in ASM-1 liquid medium sparged with sterile air and maintained under conditions of maximum asepsis, controlled temperature around 24 °C under fluorescent lamps, with a 12h light-12 h dark photo period. The C/F/DAF process was carried out with a solution of 1 % moringa seeds at concentrations varied in the range of 25 - 300 mg.L-1. Biomass lipid was evaluated by lipid composition of esters in transesterification/etherification reaction, adding to the oil, a solution of hexane, chloroform and hydrochloric acid. The esters produced were diluted in a solution of hexane and tricosanoic acid methyl ester as the internal standard in gas chromatographic analysis. Lipid material was characterized as the profile of the major fatty acid. Biomass showed a composition of palmitic acid (23.67 %), oleic acid (24.08 %) and linoleic (12.52 %). These results demostrated that cyanobacterium lipids from M. protocystis have interesting properties for biodiesel production.