Methicillin-resistant Staphylococcus aureus (MRSA) has been increasingly spreading in hospitals. The discovery of a new antibiotic is increasingly difficult due to its huge amount of time and money requirements. Medicinal herbs were used in the treatment of infectious diseases for a long time. Binh Duong province has a rich source of indigenous plants. Traditional remedies of these plants have been used in treating infectious diseases. However, there are very few studies on natural antibiotics in the area. The main purpose of this study is to investigate antibacterial activity of Syzygium glomerulatum collected in Binh Duong on methicillin-resistant Staphylococcus aureus. Agar disc diffusion and micro dilution methods were performed to determine antibacterial activity against MRSA of Syzygium glomerulatum and its ability to combine with vancomycin antibiotic. In addition, the cell toxicity of Syzygium glomerulatum was determined by SRB (Sulforhodamine B). Results showed that the minimum inhibitory concentration (MIC) of Syzygiumit glomerulatum was 2.85714 µg/mL. Meanwhile, at the concentration of 35 times MIC, Syzygium glomerulatum did not give toxicity on hepatic cancer cells G2 and fibroblast cells. The FIC index of combination of Syzygium glomerulatum and vancomycin was 0.53544, indicated a high probability of partial combination of Syzygium glomerulatum and vancomycin. Furthermore, using disc diffusion method and measuring their zone of inhibition also indicated the possibility of combination of Syzygium glomerulatum and vancomycin against MRSA strains.