Pilot-scale Operation of a Multi-enzymatic Cascade Reaction in a Multiphase System
Johannsen, Jens
Engelmann, Claudia
Liese, Andreas
Fieg, Georg
Bubenheim, Paul
Waluga, Thomas
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How to Cite

Johannsen J., Engelmann C., Liese A., Fieg G., Bubenheim P., Waluga T., 2020, Pilot-scale Operation of a Multi-enzymatic Cascade Reaction in a Multiphase System, Chemical Engineering Transactions, 79, 25-30.
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Abstract

This work aims to emphasize the importance of scale-up in biotechnological research, in order to introduce more environmentally friendly bio-catalyzed processes to the industry. Here, a complex biotechnological reference reaction system is investigated, and, for the first time, a successful synthesis of cinnamyl cinnamate from cinnamyl aldehyde in a multiphase system is established. A multi-enzymatic cascade reaction sequence with integrated co factor regeneration and in situ intermediate separation is applied. This highly integrated reaction system is successfully implemented in a pilot-scale reactor plant. Experimental results show complex relationships between physically separated unit operations.
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