The presence of biofilms in milk industry may be lead to deterioration in quality of dairy products and a risk to consumer health. The main purpose of the article is to evaluate biofilm formation ability in the 96-well microtiter plate of bacterial strains isolated from raw milk samples of cow-milk farms in BenTre province, Mekong Delta - Vietnam and identify isolates as strong biofilm formers. A total of 10 bacterial isolates is isolated from 8 raw milk samples and studied for their capabilities to form biofilm. All the isolates had biofilm-forming ability. 3, 1 and 6 of them can be classified as strong, moderate and weak biofilm producers. Among the strong biofilm formers, the BT17, BT119 isolates were 100%, 98% of identity Pseudomonas oryzihabitants strain 2.1, Serratia marcescens NPK2 2 20 respectively. The BT31 isolate had the strongest biofilm ability, extracellular proteolytic and lipolytic enzymes activities and was Aeromonas allosaccharophila BT31.