In Viet Nam, antibiotic is an important class of drugs because infectious diseases in Viet Nam is the top ratio of infections and death. Methicillin-resistant Staphylococcus aureus (MRSA) accounted for 82.1% of all isolates of S. aureus as the cause of death related to hospital-acquired pneumonia. Developing towards our ongoing studies of screening anti-Staphylococcus aureus activity of several local plants, we herein revealed the anti-MRSA activity of the extract of Carallia brachiata L. cultivated in Vietnam. The dilution method was performed to confirm the MIC value of fractions that contained anti-MRSA activity. The capacity of Carallia brachiata L. extract to inhibit MRSA’s hemolytic properties was determined by culturing MRSA on blood agar plates. The fractions from the ethanol Carallia brachiata L. extract were collected using liquid-liquid phase separation. According to the results, the minimum inhibitory concentration (MIC) value of ethyl acetate Carallia brachiata L. extract fraction against MRSA is approximately 2.5 mg/mL. The hemolytic capacity of MRSA is inhibited by ethyl acetate Carallia brachiata L. extract fraction. The potency of anti-MRSA activity of Carallia brachiata L. extract is reported for the first time.