This study investigates the sulfuric acid hydrolysis process to improve the efficiency of paper waste sludge (PWS) hydrolysis in a paper recycling plant. The Response Surface Methodology (RSM) was used to evaluate the influence of factors, including liquid-to-solid ratio, acid concentration, temperature, and time on the hydrolysis process. The optimal conditions for hydrolysis were determined to be a solid-to-liquid ratio of 1/30.15, an acid concentration of 8.17 wt%, at a temperature of 91.31 ?, and a reaction time of 6.15 h, which yielded the highest sugar content of 11.12 g.L-1 (with the hydrolysis efficiency of 61.4 %). The resulting sugar solution was then fermented by Acetobacter xylinum bacteria to produce bacterial cellulose (BC) layers. This study addresses the environmental organic pollution caused by PWS and transforms it into a more valuable form with high carbon content and purity, such as BC.