Global urbanization has contributed to heightened carbon dioxide (CO2) emissions due to extensive cement production for construction purposes. This research explores the viability of biochar as a means of sequestering CO2. Biochar, a solid material derived from the thermochemical conversion of biomass in a low-oxygen environment, exhibits promising carbon capture capabilities. The primary objective of this study is to develop a concrete mixture design incorporating chemically-activated bamboo biochar as a partial replacement for cement and to assess its capability in capturing and sequestering CO2. Bamboo biochar was produced at 300 °C and activated using sodium hydroxide. Cement replacement levels of 2 %, 5 %, and 8 % by weight were employed. Cylindrical concrete samples were subjected to compressive testing at 7 and 28 days, while the ability of biochar concrete to adsorb CO2 was evaluated using an MQ135 gas sensor test. The carbonation depth was determined after subjecting the specimens to a one-month exposure to CO2. The compressive strength test results confirmed that the 7-day strength of biochar-incorporated concrete was minimally below the standard strength while the 28-day strength was significantly higher. Meanwhile, the gas sensor test affirmed the effectiveness of biochar concrete in adsorbing CO2, as evidenced by the increase in carbonation depth. These results indicate the potential of bamboo biochar as partial cement replacement in concrete to sequester CO2 without majorly affecting its compressive strength.