Chitosan nanoparticles are a naturally derived substance with exceptional physicochemical properties and non-toxic bioactivity, making them suitable for extensive applications. This study evaluates and compares chitosan nanoparticles and chitosan nanoparticles incorporating antimicrobial agents. Copper ions were incorporated into chitosan matrices using a modified ionic gelation method to form a nano-coating solution. The particle size of chitosan nanoparticles (CN) was 118.23 ± 0.58 nm, and the particle size of chitosan nanoparticles loaded copper ions (CNCu) was 166.65 ± 7.36 nm, with low polydispersity index values and moderate to high zeta potential values. Scanning Electron Microscope (SEM) and Transmission Electron Microscopy (TEM) were used to determine their particle size and morphology. The antioxidant activity of chitosan nanoparticles and chitosan nanoparticles loaded with copper ions was also evaluated. CNCu exhibited superior antimicrobial activity against yeasts and molds. The results suggest CN and CNCu have great pre and post-harvest fruit preservation potential.