The study utilized soybean and corn residue by-products to produce bioethanol through separate saccharification and fermentation processes. The saccharification process of soybean and corn residue liquid was carried out with a-amylase enzyme, and the fermentation process of the saccharification solution used Saccharomyces cerevisiae yeast. The reducing sugar content and ethanol content of the products were determined by high-performance liquid chromatography analysis. The saccharified product contained the highest reducing sugar content of 90.09 g/L when using 7.5 g/L of a-amylase enzyme for 4 h of saccharification at 65 °C. The fermented product achieved the highest ethanol content of 5.56 vol% with the yeast content used at 6 g/L for 96 h of fermentation at 30 °C. Synthesizing bioethanol from soybean and corn residue by-products not only contributes to reducing the consumption of petroleum resources but also to environmental protection.
