Peters T., Teetz N., Bildik B.C., Hartmann P., Kraut M., Holtmann D., Dittmeyer R., 2026, A Continuous Tandem Catalytic Platform Linking Direct H2O2 Synthesis to Yeast Surface-Displayed Peroxygenases, Chemical Engineering Transactions, 124, 103-108.
Continuous tandem catalysis enables the direct coupling of chemical hydrogen peroxide (H2O2) synthesis with oxidative biocatalysis while avoiding external peroxide addition. In this study, a continuous-flow tandem system combining palladium-catalyzed H2O2 direct synthesis with yeast surface–displayed unspecific peroxygenases (YSD-UPOs) is presented. Peroxygenases were immobilized via a recently published surface-display on Komagataella phaffii cells, which were solvent-inactivated, and freeze-dried to allow application in standard chemical labs. A palladium-based catalyst was prepared on titanium dioxide as a support and evaluated for H2O2 generation under conditions compatible with enzymatic reactions. Chemical and enzymatic steps were first studied separately to identify suitable operating windows for 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) oxidation and hydroxylation of 1,2-(methylenedioxy)-4-nitrobenzene (NBD). The combined system was then operated in a continuous micro fixed-bed reactor proving the concept of continuous tandem catalysis reaching turnover numbers of 3596 for NBD and 6350 for ABTS oxidation.
