De Cata D., Mazzeo L., Piemonte V., 2026, Combined Growth Kinetics and Scale-Up Criteria for Designing CHO Cell Bioreactors, Chemical Engineering Transactions, 124, 301-306.
The scale-up of Chinese Hamster Ovary (CHO) cell bioprocesses presents significant challenges, particularly in ensuring uniform and adequate oxygen transfer throughout the reactor, while simultaneously limiting shear stress generated by the impellers, to which CHO cells are highly sensitive. This work proposes a practical and integrated approach that combines kinetic mathematical modeling with key scale-up design criteria, enabling the simultaneous consideration of the main constraints involved in CHO bioreactor design. The methodology not only ensures the achievement of the desired production target, but also supports optimal reactor design and operating conditions. The proposed framework was demonstrated through a case study, proving its robustness and reliability.
